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Lentivirus Production Services

Lentivirus Packaging Services, Custom Lentivirus Services
Catalog No. Product Name Size List Price (US$) Quantity
BS084A Molecular Construction of Lentiviral Vector and Mini-scale Production of Lentivirus 1 package 950.00
BS084B Mini-scale Production of Lentivirus 5 ml, 1 x 10^5-6 TU/ml 750.00
BS084C Midi-scale Production of Lentivirus 100 ul, 1 x 10^8-9 TU/ml 990.00
BS084D Maxi-scale Production of Lentivirus 1 ml, 1 x 10^8-9 TU/ml 7500.00
Description

Our complete custom lentivirus production services include: 1) lentivirus vector construction for expression or silencing of a gene; 2) lentivirus packaging; 3) lentivirus amplification; 4) lentivirus purification; 5) lentivirus titering.

Recombinant lentiviral vectors (rLV) are widely used in gene transfer and genome editing due to the following advantages:

- lentiviral vectors can accommodate long sequences;
- lentiviruses seem to be non-immunogenic due to the lack of viral coding sequence transfer;
- lentiviruses are able to transduce non-dividing cells;
- proteins can be stably expressed due to integration into the cell chromosome.

Our lentivirus production team has constructed, packaged, amplified, and purified thousands of lentiviruses from small crude scale to large purified scale for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate lentiviral vector construction for researchers studying gene expression or gene silencing in mammalian systems.

BS084A. Molecular construction of a lentivirus vector and mini-scale lentivirus production.
Your sequence is inserted into the lentivirus genome and transduced into mammalian cells.

Fast turnaround:
2-3 weeks.

Deliverable:
Lentivirus stock (5 ml, 1 x 10^5-6 TU/ml).

References:

Lentivirus production protocol
A polyethyenimine (PEI) transfection protocol used to produce lentivirus from a lentiviral vector transfected into Lenti-X 293T cells.

Lentivirus production and transfection protocol
A prototol for shRNA lentivirus production using HEK293T cells and FuGENE.

Protocol for generation of lentivirus
A standard lentivirus generation protocol.

The procedure of lentivirus production sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provide comprehensive lentivirus production services if the project is difficult for you.

(Disclaimer:
We index the lentivirus production protocols for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described here.)

Syd Labs provides the following lentivirus-related services:

Virus Titer Test Services for Lentiviruses and Retroviruses
Lentiviral Based Stable Cell Line Generation Services
Knock-out and Knock-in Cell Line Generation with CRISPR Genome Editing
Chimeric Antigen Receptor T-Cell Engineering

Syd Labs also provides the following virus production services (virus vector construction, virus packaging, virus amplification, virus purification, and virus titering):

Retrovirus Production Services
Adeno-associated Virus (AAV) Production Services
Adenovirus Production Services
Adenovirus Production Services for Toxic Genes
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35

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