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IgG2a Reference Antibody

Recombinant Mouse IgG2a Reference Antibody
Catalog No. Product Name Size List Price (US$) Quantity
PA007129 Recombinant Mouse IgG2a Reference Antibody 1 mg 1800.00
Description

Background

Naturally synthesized and secreted by plasma B cells, the immunoglobulin G (IgG) antibody is the most abundant antibody isotype found in blood and extracellular fluid. There are four IgG subclasses (IgG1, IgG2a, IgG2b, and IgG3) in mice, of which IgG1 is the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

The arms of the Y, also called the Fab (fragment, antigen-binding) region containing an identical antigen binding site, is composed of one variable (located at amino terminal end, VH and VL) and one constant domain (CH1 and CL) from each heavy and light chain of the antibody. VH and VL, also called the FV region, is the most important region for binding to antigens. Three variable loops of β-strands, also called the complementarity determining regions (CDRs or idiotypes), on VH and VL respecetively are responsible for binding to the antigen.

The base of the Y, also called the Fc (Fragment, crystallizable) region and composed of two heavy chains with two or three constant domains depending on the class of the antibody, binds to a specific class of Fc receptors and other immune molecules, such as complement proteins, to induce an appropriate immune response for a given antigen. The Fc region bears two highly conserved N-glycosylation sites, one on each heavy chain. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6-linked sialic acid residues.

PA007129: Recombinant Mouse IgG2a Reference Antibody

The recombinant mouse IgG2a reference antibody was produced in HEK 293 cells.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Other Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Reference Antibody
Recombinant Human IgG2 Reference Antibody
Recombinant Human IgG4-S228P Reference Antibody
Recombinant Mouse IgG1 Reference Antibody
Recombinant Mouse IgG2b Reference Antibody

Recombinant IgG Fc Proteins:
Recombinant Human Fc Protein (IgG1)
Recombinant Human Fc Protein (IgG2)
Recombinant Human Fc Protein (IgG4)
Recombinant Mouse Fc Protein (IgG1)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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