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High Yield Taq DNA Polymerases

Taq Polymerase Enzymes, Taq Enzymes, Taq Pol, Taq
Catalog No. Product Name Size List Price (US$) Quantity
MB016-HYT-S HY Taq DNA Polymerase 1000 units 90.00
MB016-HYT-M HY Taq DNA Polymerase 5000 units 350.00
MB016-HYT-L HY Taq DNA Polymerase 10000 units 600.00
Description

MB016-HYT: High Yield Taq DNA Polymerase

The high yield Taq DNA polymerase is an optimized blend of the recombinant Taq DNA polymerase (catalog No. MB042-EUT) and a proofreading enzyme with 3′→5′ exonuclease activity. The high yield Taq DNA polymerase has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.

Unit definition: One unit of the high yield Taq DNA polymerase means incorporating 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74°C.

Concentration: 5 units/ul.

Quality Control: No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.

The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator.

Features of the high yield Taq DNA polymerase:
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.

Applications of the high yield Taq DNA polymerase:
* Routine PCR using genomic, viral and plasmid templates.
* High sensitivity PCR, including difficult, low copy, or “dirty” templates.
* DNA cloning and subcloning.
* High throughput PCR.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.
* Long PCR (up to 10 kb human genomic and 15 kb lamda DNA fragments tested).

The high yield Taq DNA polymerase is also available in 2X high yield PCR Master Mix, with blue dye (catalog No. MB040-HY2).

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