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Guaranteed Rat Monoclonal Antibody Development Services

Rat Monoclonal Antibody Production, Custom Rat Monoclonal Antibody
Catalog No. Product Name Size List Price (US$) Quantity
BS009A ELISA Guaranteed Rat Monoclonal Antibody production 1 package 5200.00
BS009B ELISA Guaranteed Rat Monoclonal Antibody production + Standard Peptide Synthesis + Peptide Conjugation 1 package 5600.00
BS009C ELISA Guaranteed Rat Monoclonal Antibody production + Standard Peptide Synthesis + Peptide Conjugation + Antigen Affinity Purification 1 package 6300.00
BS009D ELISA Guaranteed Rat Monoclonal Antibody production + DNA Subcloning + Bacterial Expression and Purification 1 package 7700.00
BS009E ELISA Guaranteed Rat Monoclonal Antibody production + DNA Subcloning + Bacterial Expression and Purification + Antigen Affinity Purification 1 package 8400.00
Description

The most comprehensive rat monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.

BS009A: $4,600 for a standard 24-week hybridoma development program.
Phase I: Immunization and boosts of 5 rats following the standard immunization protocol.
Phase II: Fusion and ELISA screening. Out of 1,200 to 3,000 cells screened, about 300 microliter supernatants from 10 ELISA positive cells will be sent to customers for evaluation.
Phase III: Subcloning and expansion. All clones selected by the customer will be subcloned by limiting dilution, isotyped, and expanded.

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.

Deliverable:
* 50 ul of pre-immune serum;
* About 200 ul supernatant from 10 ELISA positive cells for evaluation;
* 1 positive hybridoma cell lines;
* Remaining antigens;
* ELISA results.

The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA > 1:40,000.

Clients have the choice for licensing antibodies to save cost and even make money.

The prices will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Mouse monoclonal antibody production service for modified peptide: +$3,000 for phospho-specific, +$3,000 for methylation-specific, +$3,000 for acetylation-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$150;
e. Protein: DNA subcloning +$250, bacterial expression and purification +$1,800;
f. Additional boost immunization for higher ELISA titer of the antisera +$150/injection;
g. Additional positive hydridoma cell line +$400;
h. Antibody purification: antigen affinity purification +$350, protein A/G affinity purification +$300, ammonium sulfate precipitation +$250;
i. Guaranteed applications: Western blot +$3,500, IP +$3,500, IF +$4,500, ICC +$4,500, IHC +$5,000.

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