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Guaranteed Mouse Monoclonal Antibody Production Services

Mouse Monoclonal Antibody Development, Custom Mouse Monoclonal Antibody
Catalog No. Product Name Size List Price (US$) Quantity
BS010A ELISA Guaranteed Mouse Monoclonal Antibody production 1 package 3900.00
BS010B ELISA Guaranteed Mouse Monoclonal Antibody production + Standard Peptide Synthesis + Peptide Conjugation 1 package 4250.00
BS010C ELISA Guaranteed Mouse Monoclonal Antibody production + Standard Peptide Synthesis + Peptide Conjugation + Antigen Affinity Purification 1 package 4600.00
BS010D ELISA Guaranteed Mouse Monoclonal Antibody production + DNA Subcloning + Bacterial Expression and Purification 1 package 5700.00
BS010E ELISA Guaranteed Mouse Monoclonal Antibody production + DNA Subcloning + Bacterial Expression and Purification + Antigen Affinity Purification 1 package 5950.00
Description

The most comprehensive mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.

BS010A: $3,900 for a standard 24-week program following the standard protocol of mouse monoclonal antibody development.
Phase I: Immunization and boosts of 5 Balb/c mice following the standard immunization protocol and ELISA screening of mouse polyclonal sera.
Phase II: Fusion of splenocytes to mouse myeloma cells and ELISA screening to isolate antigen-positive hybridoma cells from an initial count of approximately 3000 fusion clones. Supernatant (conditioned media) from 10 of the final 2-3 ELISA-positive clone population pools will be sent to clients for evaluation of specific applications.
Phase III: Subcloning of the ELISA-positive clone population pools by limited dilution to isolate individual hybridoma clones of interest, and expansion of the individual hybridoma clones. 

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.

Deliverable:
* About 200 ul supernatant from 10 ELISA positive cells for evaluation;
* 1 positive hybridoma cell lines;
* Remaining antigens;
* ELISA results.

The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA titer > 1:40 000.

Clients have the choice for licensing antibodies to save cost and even make money.

The prices will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Mouse monoclonal antibody production service for modified peptide: +$3,000 for phospho-specific, +$3,000 for methylation-specific, +$3,000 for acetylation-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$150;
e. Protein: DNA subcloning +$250, bacterial expression and purification +$1,800;
f. Additional boost immunization for higher ELISA titer of the antisera +$150/injection;
g. Additional positive hydridoma cell line +$400;
h. Antibody purification: antigen affinity purification +$350, protein A/G affinity purification +$300, ammonium sulfate precipitation +$250;
i. Guaranteed applications: Western blot +$3,500, IP +$3,500, IF +$4,500, ICC +$4,500, IHC +$5,000.

Syd Labs also provides other monoclonal antibody development services:

Rat monoclonal antibody development services
Camel monoclonal antibody development services

After great hybridoma clones have been developed, if clients want to file patents, protect the antibodies using the digital format, or develop antibody drugs, clients can ask us to clone and sequence the hybridoma antibody variable regions (VH and VL), do transient expression of chimeric antibodies (scFv or complete IgG) in CHO or HEK293 cells, or generate stable cell line expressing antibodies.

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