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Generation of Stable Cell Lines Expressing Antibodies

Stable Cell Line Development for Recombinant Antibody Expression and Purification
Catalog No. Product Name Size List Price (US$) Quantity
BS040A Generation of Suspension CHO Clonal Stable Cell Lines Expressing Recombinant Antibodies (Basic Service) 2 target genes 22000.00
BS040B Generation of Suspension CHO Clonal Stable Cell Lines Expressing Recombinant Antibodies (Premium Service) 2 target genes 35000.00
BS040C Generation of Suspension CHO Pool Stable Cell Lines Expressing Recombinant Antibodies 2 target genes 14000.00
BS045C 5' RACE-based Cloning and Sequencing of Antibody VH and VL + 0.03 Liter Production and Purification of Reconstructed Whole IgG 1 package 3400.00
BS045B 5' RACE-based Cloning and Sequencing of Antibody VH and VL + 0.03 Liter Production and Purification of Reconstructed scFv-Fc 1 package 3400.00
Description

Stable cell lines expressing recombinant antibodies are generated by experienced scientists working on antibody and cell line development services for decades. Stable cell lines developed with our proprietary recombinant antibody expression system with multiple selectable markers is very productive in expressing target proteins.

Generation of stable cell lines expressing antibodies using CHO cells:

CHO suspension clonal stable cell lines generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to simultaneously express two individual genes of interest. Other mammalian stable cell lines, such as HEK 293 cells, can be used if requested. Transfection into mammalian cells and screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. The standard method for screening clones is ELISA. Additional fee will be charged for Western blot or cell-based assay of your interest. Our fast and predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity.

Basic service of clonal stable cell line generation for antibody production (BS040A) includes:

a. Clone selection up to 24 clones;
b. Detailed characterization of the top clone during a 0.1 liter production run (viability, cell growth, and titer measurements);
c. Banking of 15 vials of the top clone (10 million cells per vial);
d. Backup vial storage for 6 months.

Deliverable:
a. 10 vials of the highest performing clone (10 million cells per vial);
b. All purified protein from 0.1 liter production run;
c. Stable cell line development study report.

Fast turnaround: 7-8 weeks.

Premium service of clonal stable cell line generation for antibody production (BS040B) includes basic service (BS040A) and the following:

a. Subcloning the top 3 clones for a second round of selection including screening of up to 72 clones;
b. Top three clones selected from the second round and characterized for viability, cell growth, and titer measurements during a 0.1 liter production run;
c. Improved expression levels, stability, and purity compared to basic service;
d. Detailed characterization of the final banked clone during a 1 liter production run starting from thaw of a frozen vial (viability, cell growth, and titer measurements);
e. Backup vial storage for 1 year.

Deliverable:
a. 10 vials of the highest performing clone (15 million cells per vial);
b. All purified protein from all production runs;
c. Stable cell line development study report.

Fast turnaround: 12-14 weeks.

Service of pool stable cell line generation for antibody production (BS040C) includes:

Deliverable:
a. 10 vials of the final pool (5 million cells per vial);
b. All purified protein from 0.1 liter production run;
c. Stable cell line development study report.

Fast turnaround: 6 weeks.

If requested, the antibody variable regions (including both VL and VH) of hybridoma cell lines will be cloned and sequenced. Antigen-antibody binding affinity will be confirmed by transiently expressing single-chain antibodies (scFv) or whole IgGs in mammalian expression systems and testing whether the recombinant scFv or antibodies retain the immunological activities of their corresponding parent monoclonal antibodies.

Syd Labs also provides the following stable cell line generation services:

Stable Cell Line Development Services for Protein Production: development of stable cell lines for recombinant protein expression.
Stable Cell Line Development Services for Biologics Production: generation of stable cell lines for manufacturing recombinant protein or antibody drugs.
Standard Stable Cell Line Development Services: non-viral generation of adhension or suspension stable cell lines with one or two gene insertion.
Lentiviral Based Stable Cell Line Generation Services: development of stable cell lines using lentivirus.

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