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Gene Synthesis Service

Catalog No. Product Name Size List Price (US$) Quantity
BS001A Standard Gene Synthesis Service 1 bp Request
BS001B Gene Synthesis and Subcloning Service for Mammalian Cell Line Generation 1 bp 1.00
Description

Synthesize any DNA sequence of your interest.

Required materials: Desired nucleotide or amino acid sequence.

BS001A. Standard gene synthesis service:

Deliverable:

a. 4-10 ug DNA cloned in a standard plasmid pUC19 with sequence optimization, synthesis, cloning, verification by sequencing and restriction digestion;
b. E. coli strains containing the plasmids with the desired sequence;
c. Plasmid map and DNA sequence.

Price:

$0.39/bp for non-complex genes shorter than 1 kb; $0.49/bp for longer DNA synthesis.
The minimum charge is $200.
$300 to subclone any size of genes into any desired plasmid vectors.

Contact us if you want to synthesize longer than 3 kb or complex genes. We may synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.


BS001B. Gene synthesis and subcloning for mammalian cell Line generation:

Subclone the synthesized DNA fragment into the mammalian expression vector commercially available or developed by customers, ourselves or our partners. We normally use expression vectors with the zeocin selectable marker. Any DNA sequence of your interest will be synthesized with sequence optimization to meet your goals such as protein expression at high and reliable levels. With an integrated system combining gene synthesis and PCR cloning, we can produce DNA constructs of any sequence up to 12 kb in length. We may synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.

Deliverable:

a. 4-10 ug DNA cloned in a plasmid with sequence optimization, synthesis, cloning, verification by sequencing and restriction digestion;
b. E. coli strains containing the plasmids with the desired sequence;
c. Plasmid map and DNA sequence.

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