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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal G-CSF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
BP000175-C002: Recombinant Human Granulocyte colony-stimulating factor (G-CSF) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser- Phe-Leu-leu.
Predicted molecular mass: 20 kDa.
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less then 0.1 ug/ml, corresponding to a Specific Activity of 6.0x 107 IU/ mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution
Other G-CSF Proteins:
Recombinant Mouse G-CSF Protein
Recombinant Human Pegylated G-CSF Protein
Recombinant Human PEG-G-CSF Protein
Recombinant Human PEG-G-CSF Protein
Recombinant Human G-CSF Protein, His Tag
Recombinant Human G-CSF Protein, HEK
Recombinant Human G-CSF Protein, CHO
Recombinant Human G-CSF Protein
Recombinant Human G-CSF Protein
G-CSF ELISA Kits:
Mouse G-csf ELISA Kit
Human G-CSF ELISA Kit
G-CSF Antibodies:
G-CSF Monoclonal Antibody
FITC Conjugated G-CSF Monoclonal Antibody
Biotin Conjugated G-CSF Monoclonal Antibody
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