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Conditions of optimal pegylated G-CSF (PEG-G-CSF) Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
The 174-amino-acid form of human Granulocyte-Colony-Stimulating Factor (G-CSF) is more abundant and active than the longer form (180-amino-acid) and widely used in research and therapeutics. PEGylation (polyethylene glycol) significantly extends half-life of the recombinant human G-CSF protein.
BP000175-GD18: Recombinant Human Pegylated Granulocyte colony-stimulating factor (PEG-G-CSF) Protein
Source: E. coli-derived.
Pegylated Recombinant Human Granulocyte colony-stimulating factor (PEG-G-CSF) Protein is a single non-glycosylated polypeptide chain containing 175 amino acids with a molecular mass of 38,800 Daltons.
The ED50 of PEG-G-CSF protein, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells, is < 0.1 ng/ml, corresponding to a specific activity of 6.0x10^7 IU/mg. PEGylation (polyethylene glycol) significantly extends half-life of the recombinant human G-CSF protein.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% tween80, 5% mannitol, pH4.0.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution
Other G-CSF Proteins:
Recombinant human G-CSF protein
Recombinant human G-CSF protein with His-tag
Recombinant human G-CSF protein produced in HEK
Recombinant human G-CSF protein produced in CHO
Recombinant mouse G-CSF protein
G-CSF Antibodies:
G-CSF monoclonal antibody
G-CSF monoclonal antibody (FITC-conjugated)
G-CSF monoclonal antibody (Biotin-conjugated)
G-CSF ELISA Kits:
Human G-CSF ELISA kit
Mouse G-CSF ELISA kit
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