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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal EK Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
Enterokinase (EK) is a serine proteinase that exhibits specificity for the sequence (Asp)4-Lys. It is the physiological activator of trypsinogen. The native EK enzyme has a heavy chain (115 kDa) covalently linked to a light chain or catalytic subunit (35 kDa). It has been well documented that the enterokinase light chain (EK) is catalytically effective (1), and thus most studies are performed using EK.
References:
1. A. Light, P. Fonseca, The preparation and properties of the catalytic subunit of bovine enterokinase, J. Biol. Chem. 259 (1984) 13195-13198.
BP000034-GD8: Recombinant Bovine Enterokinase, Light Chain
Source: Yeast-derived.
N-terminal sequence analysis: Ile801
Predicted molecular mass: 26.3 kDa
SDS-PAGE: 31 kDa
Unit Definition: 1 IU of Enterokinase (EK) will cleave 50 ug of test substrate to 95% completion in 16 hours or less at 25°C.
Unit Assay Condition: 20 mM Tris-HCl (pH 8.0 @ 25°C), 50 mM NaCl, 2 mM CaCl2, 25 ug of an MBP fusion protein test substrate and enzyme. Incubate at 23°C.
Formulation: a 0.2 um filtered solution in Tris, and NaCl.
Usage notes:
Suggested Reaction Conditions: Cleavage of an MPB-paramyosin-ΔSal fusion protein with an enterokinase site requires 1 IU per 50 ug.
Removal: Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70°C as supplied.
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