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Rabbit Polyclonal Antibody Development Protocols

Rabbit Polyclonal Antibody Development Protocols: The procedure of rabbit polyclonal antibody development sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provides cost competitive and reliable ELISA guaranteed rabbit polyclonal antibody development services if you have no resource to make it.   Disclaimer: We index the protocols […]

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RNA Extraction Protocols (Spin Columns)

Protocols of commercially available and customized RNA extraction kits using spin columns: – SV Total RNA Isolation System Protocol (Promega)   Disclaimer: We index the protocols for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described […]

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Real Time PCR (qPCR) Protocols

Real-time PCR (qPCR) protocols: The procedure of real-time PCR (qPCR) sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provides cost competitive and reliable real-time PCR (qPCR) services if you have no resource to make it.   Disclaimer: We index the protocols for your information only. It […]

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RNA Extraction Protocols (Trizol)

RNA extraction protocols of commercially available and customized TRIzol reagents from public literatures: Life Technologies provides excellent instructions about RNA and RNA isolation. – Discussion about the best protocol for RNA extraction from whole blood. Comments from scientists who have used various commercial kits including TRIzol and RNeasy to extract RNA from whole blood. – […]

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RNA Extraction Protocols (Plants)

Protocols for RNA extraction from various plant species and plant tissues: – A Method for Extracting High-Quality RNA from Diverse Plants for Next-Generation Sequencing and Gene Expression Analyses Different from most of protocols of RNA extraction which were modified for particular crop plants or model organisms such as Arabidopsis,  this RNA extraction protocol can be […]

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Plasmid miniprep protocols (spin columns)

Most of commercial plasmid miniprep kits rely on the spin column with silica membrane. The plasmid DNAs purified can be used for routine molecular biology applications such as DNA subcloning, PCR, in vitro transcription, DNA sequencing, and DNA transfection into mammalian cells. Protocols of plasmid miniprep using spin columns: – QIAprep Spin Miniprep Kit High-Yield […]

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PCR product cloning protocols

Three mainstream strategies have been used to clone and subclone PCR-amplified DNA fragments. The traditional method is to digest the PCR products with restriction enzymes and ligate the digested DNA fragments with a digested vector. The second ligation strategy is called “TA cloning” based on the feature of ends of PCR products.  The modern cloning […]

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