Three ways to submit samples for antibody sequencing:
– Freeze the antibody producing cells, and send us a frozen vial on dry ice. If you have frozen stocks, you can directly ship the stock to us or extract RNAs from the stocks. It is unnecessary to grow new cells.
– Purify total RNAs from the antibody producing cells, and send us the purified RNAs on dry ice.
– Purify total RNAs from the antibody producing cells but do not elute the RNAs from the RNA isolation column, and send us the column with the dried RNAs at ambient temperature or with ice pack. It is highly recommended to international clients.
The number of 100,000 cells is sufficient for sequencing antibodies of hybridomas or clonal B cells, i.e. the antibody producing cells mentioned below. We have also successfully sequenced antibodies in human, mouse, and rabbit single B cell and small numbers of clonal B cells.