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Antibody Production and Purification
Reagents and equipment for antibody production and purification.
- IA000043-P-9002-002
R_PE_antibody bioconjugation technology is superior to the maleimido/thiol_based method as it is:
More efficient: Greater than 95% of antibody is converted to conjugate.
More easily purified: In most cases, the percent conversion of free antibody to conjugate is 100%, leaving no unconjugated antibody. Therefore it is only necessary to purify out the excess R_PE to obtain a purified conjugate. In many applications even, purification is not necessary.
Controllable: The level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic_modification on the antibody. In flow cytometry applications, heterodimer product is required and preparation of this construct can be easily optimized using this technology.
Gentle on Antibodies: Other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. Our technology, however, gently incorporates HyNic moieties on the intact antibody. - IA000046-S-9002-1
The S-HyNic Conjugation Kit can be used to covalently crosslink any two amine containing biomolecules - IA000049-S-9003-1
Low price, high quality - IA000044-B-9007-009K
ChromaLink One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple ? 280 and ? 354 measurement determines the number of biotins per antibody molecule. Highlights:
* Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody
* Fast and easy method to biotinylate and determine the amount of labeling
* Label and determine the extent of biotin labeling with one reagent
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample - IA000045-B-9014-009K
The ChromaLink? Digoxigenin One-Shot antibody labeling kit is a convenient, cost-effective way to incorporate a digoxigenin label into a single 100 ug quantity of antibody, all in about 90 minutes. Highlights:
?Complete Kit, contains sufficient material to DIG label 100 ?g of Antibody
?Fast and easy method to DIG label and determine the amount of labeling
?Label and determine the extent of DIG incorporation with one reagent
?Simple calculations based on UV absorbance estimates total DIG incorporation (moles of DIG per mole of protein/peptide
?Nondestructive assay does not destroy DIG labeled sample - IA000047-S-9011-1
High conversion rates, coupled with the unique UV traceable bond formed during crosslinking, allows for easy purification and identification of the conjugate from the excess oligo using size exclusion purification methods such as FPLC or diafiltration. - IA000048-S-9010-1
High conversion rates, coupled with the unique UV traceable bond formed during crosslinking, allows for easy purification and identification of the conjugate from any excess protein using size exclusion purification methods such as FPLC or diafiltration. - IA000050-S-9001-1
SHNH (HyNic) is a heterobifunctional linker that reacts with primary amines on biomolecules and converts them to 4-HyNic linker groups - IA000051-S-3034
6-Fmoc-Lysine-(6-Boc-HyNic) is used to incorporate HyNic (6-hydrazinonicotinamide) moieties on the C-terminus and internal positions during solid or solution phase peptide synthesis - IA000052-HL-1002
Used for incorporation of HyNic linkers on silica/glass surfaces
89 Antibody Production and Purification Products
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