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Home > Services > Antibody Services > Antibody Engineering Services
Antibody variable region cloning and sequencing, recombinant IgG and scFv-Fc construction and production, antibody humanization, and antibody optimization.
    10 Antibody Engineering Services
  • 100000 cells per hybridoma or clonal B cells are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL). Welcome to challenge us with lower number of cells or non-viable cells for hybridoma rescue. (Higher fees may be charged. Please ask for a quotation.)

    Fast turnaround:

    * 2-3 weeks (1-2 weeks for express service) for cloning and sequencing antibody variable regions (VH and VL) of hybridoma or clonal B cells. An industry record: 4 days.
    * (Optional but highly recommended) 3-4 weeks for molecular construction, transient expression, and protein A purification of recombinant IgG or scFv-Fc from HEK 293 cells.
    * (Optional but highly recommended) 1 week for assessment of binding affinity and specificity of recombinant IgG or scFv.

  • Fast turnaround (BS079A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant scFv-Fc antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant scFv-Fc antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS079D: 0.03 liter transient production, estimated yield 0.5 - 12 mg, average 1.5 mg, 2 weeks;
    * BS079E: 0.1 liter transient production, estimated yield 3 - 110 mg, average 14 mg, 2 weeks;
    * BS079F: 0.5 liter transient production, estimated yield 12 - 300 mg, average 75 mg, 2 weeks;
    * BS079G: 2 liter transient production, estimated yield 50 - 2000 mg, average 250 mg, 3 weeks;
    * BS079H: 10 liter transient production, estimated yield 120 - 5000 mg, average 1100 mg, 3 weeks.

  • Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

    A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

    Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.

  • Fast turnaround: 3-6 months with our proprietary optimization algorithm.

    Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.

  • Fast turnaround (BS046A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant IgG antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant IgG antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS046D: 0.03 liter transient production, estimated yield 0.5 - 10 mg, average 2 mg, 2 weeks;
    * BS046E: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
    * BS046F: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
    * BS046G: 2 liter transient production, estimated yield 40 - 2000 mg, average 200 mg, 3 weeks;
    * BS046H: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.

  • It is sometimes desirable but always challenging to clone and sequence the antibody variable region (VH and VL) sequences from single B cells. Syd Labs has developed methods to isolate and amplify RNA from single B cells, enabling identification of the VH and VL sequences in single B cells.

  • Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.

  • Syd Labs screens scFv phage libraries using several different formats for applications including de novo antibody discovery and affinity maturation of existing antibodies.

  • Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.

  • Our epitope binning service include: Antigen is immobilized onto a biolayer interferometry (BLI) sensor using an appropriate method. Antibody 1 is allowed to saturate its epitope on the antigen. Binding of Antibody 2 to antigen saturated with Antibody 1 is assessed.

    Fast turnaround: 2-3 weeks.

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