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Antibody Digestion to Fab or F(ab)2 Fragments

Name: Antibody Digestion to Fab or F(ab)2 Fragments
Brand: Syd Labs
SKU: BS037A
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.	Product Name	Size	List Price (US$)
BS037A	Analytical Digestion by Pepsin	1 mg	Request
BS037B	Analytical Digestion by Papain	1 mg	Request
BS037C	Digestion of 10 mg Antibody by Pepsin	10 mg	Request
BS037D	Digestion of 10 mg Antibody by Papain	10 mg	Request

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Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Description

We use pepsin for F(ab')2 fragment production and papain for Fab fragment production. Before digesting large amount of antibodies, we perform an analytical digestion to optimise conditions. Undigested antibodies will be removed by protein A columns.

Customers should provide minimum of 10 mg antibodies. Normally 3 mg of Fab or F(ab')2 fragments can be obtained from 10 mg antibodies after absorption with Protein A. The resulted fragments should remain their binding abilities to antigens and show one band (about 100 kDa for F(ab')2 fragments and 50 kDa for Fab fragments) under non-reducing conditions and two bands (both are around 25 kDa) on SDS-PAGE under reducing conditions.

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Antibody Digestion to Fab or F(ab)2 Fragments

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