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Albumin Fusion Protein Production Services

Expression and Purification of Albumin Fusion Proteins
Catalog No. Product Name Size List Price (US$) Quantity
BS074A Molecular Construction of Expression Vector for Albumin Fusion Protein + 1 mg Endotoxin-free DNA production 1 construct 1400.00
BS074B Molecular Construction of Expression Vector for Albumin Fusion Protein + 0.1 liter Transient Production of Albumin Fusion Protein 1 package 2700.00
BS074C 0.03 Liter Transient Production of Albumin Fusion Protein 0.03 liter 1700.00
BS074D 0.1 Liter Transient Production of Albumin Fusion Protein 0.1 liter 2200.00
BS074E 0.5 Liter Transient Production of Albumin Fusion Protein 0.5 liter 4900.00
BS074F 2 Liter Transient Production of Albumin Fusion Protein 2 liter 9500.00
BS074G 10 Liter Transient Production of Albumin Fusion Protein 10 liter 31200.00
Description

Syd Labs has extensive experience with making DNA constructs for albumin fusion protein production, expressing and purifying a number of albumin fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized expression and purification process, Syd Labs can efficiently complete a project from DNA construction and transient production of albumin fusion proteins in CHO and HEK 293 cells, to albumin fusion protein purification.

Proteins destined for in vivo use can be fused to albumin to improve plasma half-life and bioavailability due to decreased renal clearance and recycling via the neonatal Fc receptor (FcRn). Different from Fc fusion proteins, albumin fusion proteins are monomeric and do not interact with Fc receptors.

1. DNA construction of albumin-fused proteins:

The gene of interest is synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors. If gene synthesis is requested, free sequence optimization is performed to meet the client's goals such as recombinant albumin-fusion protein expression at high and reliable levels.

2. Expression and purification of albumin-fusion proteins:

* Transiently express the recombinant albumin-fusion protein in pilot small-scale (100 ml) of CHO or HEK 293 cells, followed by custom albumin affinity purification, to assess expression levels and protein yield;
* If requested, scale up transient expression to 0.25 - 20 liters to produce starting material at the milligram to gram scale;
* Purification is performed using a custom albumin affinity purification process. Please request if multiple purification methods are required.

If you provide us the albumin fusion protein expression vector with your gene already cloned into, we perform small-scale pilot expression and protein purification by transient transfection into CHO or 293 cells, allowing us to evaluate expression level and yield of the albumin fusion protein of your interest.

Fast turnaround (BS074A):
* 1-2 weeks for codon optimization and synthesizing the gene of your interest.
* 1-2 weeks for molecular construction of recombinant albumin fusion proteins using E.coli.

We also provide the following protein production (DNA construction, protein expression and purification) services:
Fc Fusion Protein Production Service
His Tag or FLAG Tag Fusion Protein Production Service

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