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Adeno-associated Virus Production Services

AAV Production Services, AAV Packaging Services
Catalog No. Product Name Size List Price (US$) Quantity
BS090A Molecular Construction of AAV Vector and Production of AAV 1 package 3990.00
BS090B Mini-scale Production of AAV 0.1-0.2 ml, 5 x 10^12 GC/ml 490.00
BS090C Midi-scale Production of AAV 0.5-1 ml, 5 x 10^12 GC/ml 1990.00
BS090D Maxi-scale Production of AAV 1.5-2 ml, 5 x 10^12 GC/ml 3490.00
Description

Our complete custom Adeno-associated Virus (AAV) production services include: 1) AAV vector construction for protein expression or gene silencing; 2) AAV packaging; 3) AAV amplification; 4) AAV purification; 5) AAV titering.

Recombinant AAV (rAAV or AAV below) vectors are currently among the most frequently used viral vectors for gene therapy. AAV is ideal to be used as the vehicle for gene transfer and the tool for genome editing because:

- AAV is simple and easily manipulated;
- AAV can deliver genes to both dividing and non-dividing cells, and a wide variety of tissue types from muscle to brain;
- AAV is lack of pathogenicity and elicits very low immunogenicity only;
- AAV allows expression of proteins in host cells for long period of time.

Our professional AAV production team members have successfully produced high quality AAV (conventional or double-stranded also called self-complementary) with high titer for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate AAV vector construction for researchers studying gene expression and silencing in mammalian systems.

BS090A. Molecular construction of an AAV vector and maxi-scale AAV virus production.
Your sequence is inserted into the AAV genome and transduced into mammalian cells.

* Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV7, AAV8, AAV9).
* Wide choice of AAV cis vectors (promoterless, CMV, PGK, UBC or U6 promoters with or without reporter genes like GFP, mCherry, strawberry, Luc, LacZ, etc).
* From small crude scale to large purified scale. For example, midi-scale AAV transfection of HEK 293 cells in 20 x 150 mm plates.
* Harvest AAV followed by purification via a serial of Iodixanol or CsCl centrifugation.
* AAV titering (measuring genome copies).

Fast turnaround:
2-3 weeks.

Deliverable:
1.5-2.0 ml purified viral stocks (5 x 10^12 GC/ml). Final viral yield may depend on the nature of transgene.

References:

A protocol for AAV vector production and purification
A method used to propagate and purify AAV vectors for experiments both in vitro and in vivo.

Production and Titering of Recombinant Adeno-associated Viral Vectors
A method for the production and titering of chimeric rAAVs containing the capsid proteins of both AAV1 and AAV2.

Inexpensive, serotype-independent protocol for native and bioengineered recombinant adeno-associated virus purification
A standard AAV virus purification protocol independent of toxic compounds, supernatant volume and capsid moiety, and across native serotype and bioengineered mosaic capsids.

The procedure of AAV production sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provide comprehensive AAV virus production services if the project is difficult for you.

(Disclaimer:
We index the AAV production protocols for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described here.)

Syd Labs also provides the following virus production services (virus vector construction, virus packaging, virus amplification, virus purification, and virus titering):

Retrovirus Production Services
Lentivirus Production Services
Adenovirus Production Services
Adenovirus Production Services for Toxic Genes
Adenovirus Production Services for Fiber-modified Ad5-RGD and Ad5/35

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