BP000027-GD1: Recombinant Human VEGF165 (aa 27-191) Protein
Source: HEK 293 cell-derived.
The VEGF activity is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/ml.
Endotoxin: < 1.0 EU per ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions.BP000035-GD9: Recombinant Human FSH α/β Protein
Source: CHO cell-derived.
The FSH α/β activity is measured in a human FSH receptor transfected HEK 293 cells. The ED50 for this effect is 0.1-0.4 ng/ml.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.BP000050-GD13: Recombinant Human RANKL Protein
Source:E. coli-derived.
The RANKL / TNFSF11 / TRANCE / OPGL / CD254 activity is calculated by its ability to induce osteoclast differentiation on RAW 264.7 mouse macrophages. The ED50 for this effect is 2-12 ng/ml.
Endotoxin Level: <1.0 EU per ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE and SEC-HPLC.BP001573-K0104: Recombinant Human PAK1 Kinase Domain (PAK1KD) Enzyme
Source: E. coli-derived.
Specific activity: 32,638 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.BP001574-K0105: Recombinant Human PAK2 kinase domain (PAK2KD) Enzyme
Source: E. coli-derived.
Specific activity: 85,806 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.BP001575-K0107: Recombinant Human PAK4 Kinase Domain (PAK4KD) Enzyme
Source: E. coli-derived.
Specific activity: 5,595 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.BP001576-K0109: Recombinant Human PAK6 Kinase Domain (PAK6KD) Enzyme
Source: E. coli-derived.
Specific activity: 2,086 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.BP001577-K0108: Recombinant Human PAK7 Kinase Domain, aka PAK5 (PAK7KD) Enzyme
Source: E. coli-derived.
Specific activity: 4,199 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.BP001020-Z65: Recombinant Human transcription factor p65 protein
Source: Baculovirus, SF9 insect cells-derived.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 90%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method.BP000175-GD16: Recombinant Human Granulocyte colony-stimulating factor (G-CSF) Protein
Source: E. coli-derived.
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is < 0.1 ng/ml, corresponding to a specific activity of 6.0x10^7 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.01 ng/ug (0.1 IEU/ug).