BP000029-GD3: Recombinant Human FGF Acidic 136 (aa 19-155) Protein
Source: E. coli-derived.
The FGF acidic (aFGF/FGF-1/FGF1) activity is measured in a NIH/3T3 cell proliferation assay. The ED50 for this effect is typically 0.1-0.3 ng/ml in the presence of 10 ug/ml of heparin.
Endotoxin: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.BP000031-GD5: Recombinant Heparinase I from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >100 IU/mg. One international unit (IU) of recombinant Heparinase I is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0.
Purity >95% by SDS-PAGE.BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.BP000033-GD7: Recombinant Heparinase III from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >50 IU/mg. One international unit (IU) of recombinant Heparinase III is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.BP000036-GD10: Recombinant Human FVII Protein
Source: HEK 293 cell derived.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.BP000036-GD11: Recombinant Human FVIIa Protein
Source: 293 cell derived.
The potency per mg of recombinant human FVIIa protein was tested and found to be 50,000 Units/mg.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions.BP000037-GD12: Recombinant Human FVIII Protein
Source: 293 cell-derived
The potency per mg of recombinant human FVIII protein was tested and found to be 11,000 Units/mg.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.BP000051-GD14: Recombinant Human OPG-Fc Fusion Protein
Source: HEK 293-derived.
The Osteoprotegerin / OPG / TNFRSF11B / OCIF / OBF activity is calculated by its ability to inhibit TRAIL-mediated cytotoxicity using L929 mouse fibrosarcoma cells treated with TRAIL. The ED50 for this effect is typically 5 ng/ml.
Endotoxin: < 5.0 EU per 1 ug of the protein by the LAL method.
Purity: > 90% by SDS-PAGE and RP-HPLC.BP000339-GD15: Recombinant Human Chorionic Gonadotropin (hCG)
Source: CHO-derived.
The hCG α/β activity was determined to be 25,000 IU/mg.
Purity > 97% by SDS-PAGE under reducing conditions and visualized by silver stain, and 97% by SEC-HPLC.BP002388-Z53: Recombinant Human Cellular tumor antigen p53 (p53) Protein
Source: Baculovirus, SF9 insect cells-derived.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 95%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method.