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Virus Packaging Services
Note: Price/availability/specifications subject to change without notice. If not specified, all products and services are for in vitro research use only. Not for use in or on humans or animals.
Order offline: Phone: 1-617-401-8149, Fax: 1-617-606-5019, Email: message@sydlabs.com, or leave a message with a formal purchase order (PO) or credit card.
Features:
Syd Labs provides packaging services for both DNA integrative viruses and DNA non-integrative viruses. DNA integrative viruses include retrovirus and classical lentivirus. DNA non-integrative viruses include modified lentivirus, baculovirus, adenovirus, adeno-associated virus, and baculovirus.
The viral titer and purity guaranteed effective infection to both cultured cells and tissues:
The titer of packaged retroviruses: 1x10^6 to 1x10^8 TU/ml.
The titer of packaged lentivruses: 1x10^6 to 1x10^8 TU/ml.
The titer of packaged non-integrative lentivruses: 1x10^6 to 1x10^8 TU/ml
The titer of packaged adenoviruses: 1x10^10-1x 10^12 pfu/ml.
The titer of packaged adeno-associated virus: 1x10^11-1x 10^12 vg/ml.
The titer of packaged baculoviruses: 1x10^6-1x 10^8 TU/ml.
Fast turnaround time: 1- 3 months.
Description:
BS0064-1. Retrovirus packaging service.
Retrovirus is an RNA virus that is replicated in a host cell via the enzyme reverse transcriptase to produce DNA from its RNA genome. The DNA is then incorporated into the host's genome by an integrase enzyme. The virus thereafter replicates as part of the host cell's DNA. It can effectively infect multiple mammalian cell types and thus serve as an important tool to achieve stable expression in host cells.
BS0064-2. Lentivirus packaging service.
Lentivirus is a genus of slow viruses of the Retroviridae family, characterized by a long incubation period. Lentiviruses can deliver a significant amount of genetic information into the DNA of the host cell and have the unique ability among retroviruses of being able to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. Like many other types of retroviruses, lentivirus is an excellent research tool for stable introduction of a gene product into in vitro systems or animal models for stable expression.
BS0064-3. Non-integrative lentivirus packaging service.
Non-integrative lentivirus system utlizes a modified lentiviral vector packaging helpers by inactivating the integrative elements of classical lentivruses. The modified lentivirus can efficiently infect a variety of mammalian cells like classical lentivirus whereas its viral DNA does not integrate into the host genome, thus it serve as an excellent alternative for routine transient transfection while it can also avoid the integrative side effects of classical lentivirus.
BS0064-4. Adenovirus packaging service.
Adenoviruses are medium-sized (90–100 nm), nonenveloped icosahedral viruses composed of a nucleocapsid and a double-stranded linear DNA genome. It can infect various species of vertebrates. Adenoviruses possess a linear dsDNA genome and are able to replicate in the nucleus of mammalian cells using the host’s replication machinery. Adenovirus is used as a vehicle to administer targeted therapy, in the form of recombinant DNA or protein.
BS0064-5. Adeno-associated virus packaging service.
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. Moreover, virus infection causes a very mild immune response. AAV can infect both dividing and non-dividing cells These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise. Different AAV serotypes have demonstrated patterns for the target genes in terms of expression time and host selectivity. The Biowit virus platform provides a variety of AAV2 serotypes including AAV2/1, AAV2/2, AAV2/3, AAV2/4, AAV2/5, AAV2/6, AAV2/7, AAV2/8 and AAV2/9.
BS0064-6. Baculovirus packaging service.
The baculoviruses are a family of large rod-shaped viruses and have very species-specific tropisms among the invertebrates with over 600 host species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. They are not known to replicate in mammalian or other vertebrate animal cells. Baculoviruses contain circular double-stranded genome ranging from 80–180 kbp. Thus，it widely serve not only as an excellent vehicle for overproducing the protein products in insect cells but also as an efficient transient transfection choice for introducing the gene or DNA fragment into a variety of mammalian species.
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