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Features:

PA000274-E0T503: DYKDDDDK-Tag Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: WB;
Reactivity: Human, Mouse, Rat, Others.

PA000274-E1T508: DYKDDDDK-Tag Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: WB, IF, IP;
Reactivity: Human, Mouse, Rat, Others;
Clone: G191;
Isotype: IgG2b.

PA000274-M20008: DYKDDDDK-Tag (3B9) Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: ELISA, WB, IF, IP.
Reactivity: Human, Mouse, Rat, Others.
Clone: 3B9;
Isotype: IgG1.

PA000274-P-210: DYKDDDDK-Tag Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: WB, IP;
Reactivity: Human, Mouse, Rat, Others;
Clone: Flag C2.

Description:

Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

PA000274-E0T503: DYKDDDDK-Tag Polyclonal Antibody

Immunogen: The antiserum was produced by immunizing rabbit with synthesized peptide containing the DYKDDDDK epitope.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Specificity/Sensitivity: DYKDDDDK-Tag polyclonal antibody detects DYKDDDDK-tagged proteins overexpressed in cells. The antibody recognizes the DYKDDDDK-tag fused to either the amino-or carboxy-terminus of targeted proteins in transfected mammalian cells.
Applications: WB: 1:500~1:1000

PA000274-E1T508: DYKDDDDK-Tag Monoclonal Antibody

Immunogen: A synthetic DYKDDDDK peptide coupled to KLH.
Specificity/Sensitivity: Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Applications: Western Blot 1:1000-4000, Immunoprecipitation and immunostaining 1:200-400.

PA000274-M20008: DYKDDDDK-Tag (3B9) Monoclonal Antibody

Immunogen: This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
Specificity/Sensitivity: DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.
Applications: Western blotting 1:5000, Immunofluorescence 1:1000, Immunoprecipitation 1:100, ELISA 1:2000

PA000274-P-210: DYKDDDDK-Tag Monoclonal Antibody

Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity/Sensitivity: Recognises over-expressed proteins containing DYKDDDDK epitope tag fused to either amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Formulation: 1mg/ml in PBS (pH 7.2) with 50% glycerol.
Applications: Western Blot 1:1000, Immunoprecipitation and Immunostaining 1:200.

Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Related Links:

• Magnetic Microbead Conjugated DYKDDDDK-Tag Antibody
• DYKDDDDK Peptide
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• His-Tag Antibody
• Free Antibody Samples